Journal: Pediatric Reports

Article Title: Resistin in Urine and Breast Milk: Relation to Type of Feeding and Anthropometry at 1-Month

doi: 10.3390/pediatric14010013

Figure Lengend Snippet: Correlation between resistin concentration in the breast milk and ( a ) body weight; ( b ) age; ( c ) body mass index (BMI); and ( d ) leptin concentration in mothers’ breast milk at 1 month post-partum.

Article Snippet: We analyzed urinary and breast milk resistin levels (Human Resistin ELISA kit; Arigo biolaboratories, Hsinchu City, Taiwan) and breastmilk leptin (Human Leptin Quantikine ELISA Kit; R&D Systems, Minneapolis, MI, USA) in infants using an enzyme-linked immunosorbent assay kit.

Techniques: Concentration Assay

Journal: Journal of Biological Chemistry

Article Title: Glycodelin-A Protein Interacts with Siglec-6 Protein to Suppress Trophoblast Invasiveness by Down-regulating Extracellular Signal-regulated Kinase (ERK)/c-Jun Signaling Pathway

doi: 10.1074/jbc.m111.233841

Figure Lengend Snippet: FIGURE 6. Interaction between GdA and Siglec-6. A, Western blot analysis of the interaction between recombinant Siglec-6 chimeric protein and either GdA (left) or desialylated GdA (middle). Right, the GdA protein bands were quantified by densitometry (n 3). Data were expressed as the percentage of changes relative to the normal GdA group. *, p 0.05 when compared with normal GdA group. MW indicates molecular mass markers. B, GdA-treated JEG-3 cell lysates were co-immunoprecipitated with an anti-glycodelin antibody Sepharose column. Immunoblotting was performed with anti-Siglec-6 antibody. JEG-3 cell lysate without GdA treatment served as control. C, competition binding of JEG-3 cells to Alexa Fluor 488 fluorescence dye-conjugated GdA in the presence of leptin.FlowcytometryanalysiswasdonetoquantifythebindingofGdA(n4).Valuesarepresentedasthepercentageofchangesrelativetothenotreatment group. *, p 0.05 when compared with the no treatment group.

Article Snippet: Competition Binding Assay—The binding of 33 nM Alexa Flour 488 fluorescence dye-labeled GdA to JEG-3 cells was determined in the presence of 330 nM recombinant human leptin (R&D Systems), which is a reported ligand of Siglec-6 (19).

Techniques: Western Blot, Recombinant, Immunoprecipitation, Control, Binding Assay, Fluorescence

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: Comparison of circulating leptin ( A ), FLI ( B ), sOB-R ( C ) and ghrelin ( D ) pre-surgery and post-surgery (week 1 until 12 months post-surgery). Statistically significant differences were observed in groups post-surgery, * p < 0.01; ** p < 0.001; *** p < 0.005; **** p < 0.0001.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Comparison

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: ( A ) Correlation matrix between circulating leptin, ghrelin, FLI, and anthropometric/metabolic variable changes following metabolic and bariatric surgery. Values are repeated-measures correlations (also called intra-individual correlations). Only significant results are shown based on Bonferroni-adjusted p -value. ( B ) Graph of correlations of sOB-R and FLI with leptin.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: bioRxiv

Article Title: Adipose Tissue Inflammation, Oxidative Stress, and Altered Adipogenesis Are Drivers of Dyslipidemia – A Multi-Omics Overview of Dyslipidemia in Obesity

doi: 10.1101/2025.10.15.682716

Figure Lengend Snippet: Correlation plots of leptin with HDL-C and ApoAI with lines of best fit and confidence interval. Spearman’s rank correlation (r), and its Benjamini-Hochberg adjusted p-value ( p ), only significant correlations depicted.

Article Snippet: Plasma adiponectin and leptin concentrations were measured using human Adiponectin/Acrp30 DuoSet ® ELISA (Y1065-05; R&D Systems, Minneapolis, USA) and human Leptin DuoSet ® ELISA (DY398-05; R&D Systems, Minneapolis, USA).

Techniques: