Journal: Oncology Letters

Article Title: Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer

doi: 10.3892/ol.2019.10381

Figure Lengend Snippet: Leptin reduces the chemosensitivity of ovarian cancer cells to PTX/TXT. The prognosis of patients with epithelial ovarian cancer receiving (A) PTX (P=0.008) or (B) TXT (P=0.0021) chemotherapy was analyzed based on leptin RNA expression level. CCK-8 assay was performed to assess the cell viability of HO8910PM cells treated with DDP combined with (C) PTX/ (D) TXT with or without leptin. CCK-8 assay was performed to assess the HO8910PM cell survival percentage treated with (E) PTX/ (F) TXT with or without leptin. (G) The expression of leptin was highest in the HO8910PM and OV-MZ-15 cell lines. CCK-8 assay was performed to assess the cell viability of OV-MZ-15 cells treated with DDP combined with (H) PTX/ (I) TXT with or without leptin. CCK-8 assay was performed to assess the cell viability of OV-MZ-15 cells treated with (J) PTX/ (K) TXT with or without leptin. Data are presented as the means ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001.

Article Snippet: Recombinant Human Leptin (Bio-Techne) was reconstituted at 1 mg/ml in sterile 20 mM Tris-HCl (pH 8.0) and stored at −80°C.

Techniques: RNA Expression, CCK-8 Assay, Expressing, Standard Deviation

Journal: Oncology Letters

Article Title: Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer

doi: 10.3892/ol.2019.10381

Figure Lengend Snippet: Effects of leptin on the cell cycle distribution of ovarian cancer cells following PTX/TXT treatment. Flow cytometry at (A) 8 and (B) 16 h was used to assess the effect of PTX on the cell cycle distribution of ovarian cancer cells in the presence of leptin. Flow cytometry at (C) 8 and (D) 16 h was used to assess the effect of TXT on the cell cycle distribution of ovarian cancer cells in the presence of leptin. *P<0.05, **P<0.01.

Article Snippet: Recombinant Human Leptin (Bio-Techne) was reconstituted at 1 mg/ml in sterile 20 mM Tris-HCl (pH 8.0) and stored at −80°C.

Techniques: Flow Cytometry

Journal: Oncology Letters

Article Title: Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer

doi: 10.3892/ol.2019.10381

Figure Lengend Snippet: CEBPA initiates the transcription of leptin in ovarian cancer cells. (A) A chromatin immunoprecipitation-polymerase chain reaction assay was performed for HO8910PM and OV-MZ-15 cells. Total DNA (Input), IgG and CEBPA immunoprecipitated DNA were amplified using a series of primers covering the 3,000 bp sequence upstream of the leptin transcription start site. Primer 1 and 9 flanking sequences can be immunoprecipitated with CEBPA. (B) Site directed mutagenesis of the upstream loci of leptin. Leptin vector, leptin-WT or mutant plasmids leptin-MUT1 and 2 were transfected into CEBPA overexpression or control (C) HO8910PM and (D) OV-MZ-15 cells. The pRLTK Renilla luciferase expression plasmid was co-transfected as the internal control. Luciferase activities were normalized to Renilla activities. MUT, mutant; WT, wild-type; CEBPA, CCAAT/enhancer-binding protein α; IgG, immunoglobulin G. **P<0.01, ***P<0.001.

Article Snippet: Recombinant Human Leptin (Bio-Techne) was reconstituted at 1 mg/ml in sterile 20 mM Tris-HCl (pH 8.0) and stored at −80°C.

Techniques: Chromatin Immunoprecipitation, Polymerase Chain Reaction, Immunoprecipitation, Amplification, Sequencing, Mutagenesis, Plasmid Preparation, Transfection, Over Expression, Luciferase, Expressing, Binding Assay

Journal: Oncology Letters

Article Title: Leptin contributes to the taxol chemoresistance in epithelial ovarian cancer

doi: 10.3892/ol.2019.10381

Figure Lengend Snippet: High leptin expression is correlated with a significant enrichment of multiple hallmark of cancer gene sets. (A) A total of 46 patients with ovarian cancer treated with PTX were assessed and divided into two groups according to leptin expression. ***P<0.001. (B) A heatmap of differentially expressed genes between groups associated with low leptin expression one and two was constructed group 1, leptin low expression; group 2, leptin high expression; ‘red’ means upregulation and ‘green’ means downregulation). (C) Results from the gene set enrichment analysis revealed the leptin-associated enrichment gene sets with the highest NES value. (D-I) The test leptin-associated enrichment gene sets were closely associated with tumor progression and chemoresistance and included the following hallmark: (D) HALLMARK_EPITHELIAL_MESENCHYMAL_TRANSITION; (E) HALLMARK_TNFA_SIGNALING_VIA_NFKB; (F) HALLMARK_KRAS_SIGNALING_UP; (G) HALLMARK_HYPOXIA; (H) KEGG_ECM_RECEPTOR_INTERACTION; and (I) HALLMARK_IL6_JAK_STAT3_SIGNALING NES, Normalized Enrichment Score.

Article Snippet: Recombinant Human Leptin (Bio-Techne) was reconstituted at 1 mg/ml in sterile 20 mM Tris-HCl (pH 8.0) and stored at −80°C.

Techniques: Expressing, Construct

Journal: bioRxiv

Article Title: White adipose tissue undergoes pathological dysfunction in the TDP-43A315T mouse model of amyotrophic lateral sclerosis (ALS)

doi: 10.1101/2025.07.03.662925

Figure Lengend Snippet: (A) Concentration of leptin levels at diagnosis measured by ELISA in healthy controls and ALS subgrouped by sex. (B) Concentration of leptin levels at diagnosis in ALS and controls subgrouped by sex and disease progression: slow, normal and fast progression patients. (C) Concentration of leptin levels at diagnosis in ALS and controls subgrouped by sex and rate of progression in overweight patients. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Abbreviations: ALS, sporadic amyotrophic lateral sclerosis.

Article Snippet: Leptin plasma levels were measured using a Human Leptin ELISA Kit PicoKine® (Boster Biological Technology) with samples diluted 1:20 for males and 1:10 for females.

Techniques: Concentration Assay, Biomarker Discovery, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Whole-body sorcin invalidation does not cause hypothalamic ER stress nor worsens obesity in C57BL/6 male mice fed a westernized diet

doi: 10.1101/2022.09.07.506981

Figure Lengend Snippet: (A) Representative Western (Immuno)blotting of pSTAT3 (Y705), STAT3 and Sorcin from whole protein lysate isolated from wild-type and sorcin null HEK293 cells expressing LepRb and stimulated with leptin (0-1 ng/mL) as described in methods. (B) Quantification of Western (Immuno)blotting from 3 independent experiments, showing the ratio between pSTAT3/STAT3 for cells stimulated with leptin (0-10 ng/mL); NT = non-transfected cells. Values are means ± SEM. Data were analysed for significance using 1-way ANOVA analysis with Tukey’s multiple comparisons test.

Article Snippet: HEK293 cells, seeded in 6-well plates, were transfected with 2 μg LepRb-HA plasmid, encoding full length clone DNA of Human leptin receptor, transcript variant 1 with C terminal HA tag (Sino-Biologicals, # HG10322-CY), using Xfect ™ Transfection Reagent (Takara Bio) according to manufacturer’s instructions.

Techniques: Western Blot, Isolation, Expressing, Transfection